High-density single nucleotide polymorphisms (SNP) marker panel

The USDA Agricultural Research Service is seeking high-density single nucleotide polymorphisms (SNP) marker panel services to support the Horticultural Research Lab in Fort Pierce, Florida. This requirement focuses on genotyping Florida Pompano through pooled whole genome sequencing and the development of a panel with 30,000 to 60,000 polymorphic markers. The acquisition is a total small business set-aside conducted under FAR Part 12 for commercial items, with a firm-fixed-price purchase order to be awarded based on the Lowest Price Technically Acceptable (LPTA) method. Technical specifications include PCR-free library preparation and a sequencing depth of 20X per sample. The final deliverable is a digital SNP panel design and associated data files, following a validation phase by the contractor. Quotes are due by July 10, 2026. The USDA is procuring genomic services to develop a high-density SNP marker panel for Florida Pompano genotyping. Perform whole genome sequencing with a minimum depth of 20X coverage for each individual sample.